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Fish Report, Other Fish Species, Research, Telemetry

Green sturgeon

When it comes to a threatened fish population like the Sacramento River green sturgeon (Acipenser medirostris), every adult counts–especially females. These prehistoric-looking fish have many traits that stress the value of each individual, and make their population vulnerable to environmental changes. Green sturgeon mature late, live long, grow large, reproduce multiple times, and don’t reproduce every year. The massive fish can grow 2.3 m long (7.5 ft) in the Sacramento River, can live for 50 years or more, and, similar to humans, females may not start having offspring until they are 16-27 years old (Beamesderfer et al. 2007). Losing these precious mature females can deal a serious blow to the population, and scientists have recently discovered a new threat to sturgeon on the Sacramento River: getting trapped behind flood diversions after waters recede. Green sturgeon habitat on the Sacramento River has undergone extensive modifications, including the construction of flood control structures that divert water from urban areas during floods, but also form barriers to fish migration.

Scientists didn’t realize that flood diversions posed such a danger to sturgeon on the Sacramento River until dozens of the fish got stuck out of the water during their spring spawning migrations in April 2011, prompting a rescue operation. Researchers from the UC Davis Department of Wildlife, Fish, & Conservation Biology, California Department of Fish & Wildlife (CDFW), and the U.S. Army Corps of Engineers decided to conduct a study to determine how such stranding could affect the threatened green sturgeon population in the long run, and whether rescuing the fish helped their survival or spawning success. One of the study co-authors, FISHBIO biologist Matthew Peterson, participated in the research during his previous position at UC Davis. The team’s findings, published this month in the North American Journal of Fisheries Management, indicate that rescuing trapped sturgeon can improve their survival and spawning success, but doesn’t entirely offset the negative impact of stranding on the population.

After the April 2011 flood, the researchers, personnel from the National Oceanographic and Atmospheric Administration, and many CDFW volunteers recovered 25 stranded green sturgeon at two sites on the Sacramento River, all mature adults. Of these, 23 were in good enough condition to tag and study. The scientists performed quick surgeries to implant acoustic tags and checked each fish’s sex and reproductive state before release. The team then tracked sturgeon movement using an extensive array of 47 acoustic monitors stationed along the Sacramento River, as well as a mobile tracking receiver. After release, 71% of the rescued sturgeon successfully migrated to their spawning grounds. The authors constructed a mathematical model to investigate the effects of stranding and rescue over the long term. They predicted that if stranding events occur repeatedly over the next 50 years, which they are expected to do based on flood patterns, the number of adult females would decline by a third, dealing a major blow to the viability of the population. In contrast, rescuing the stranded sturgeon would lead to a mere 7% decline over 50 years. Returning stranded fish to the main-stem Sacramento River clearly benefits the population, but saving each fish out of water is not a viable long-term strategy for protecting the population. Effectively reducing the threat of stranding will require modifying flood control structures to allow sturgeon to pass through, the authors say.

This post featured in our weekly e-newsletter, the Fish Report. You can subscribe to the Fish Report here.

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Telemetry

Using the latest technology in fisheries research often requires a reliable power supply. When practical, we utilize solar power, but some locations don’t yield themselves to extended periods of sunlight. This is the case for a Passive Integrated Transponder (PIT) tag detection system we recently installed in in a steep canyon of a Northern California watershed. For this project, we decided on a thermoelectric generator (TEG) from Global Thermoelectric. Thermoelectric generators have been available for years and have been used to power telecommunications on remote mountaintops and even spacecraft, but their application in fisheries is just beginning. The technology (see video) utilizes a temperature gradient between semiconductor materials to create direct current (DC) electricity. The system we are using runs on propane or natural gas and can be configured to produce between 6 and 48 volts. Because there are no moving parts, the TEG requires little maintenance and the DC electricity produces little electronic noise, which can be problematic for PIT tag systems. The only real limitation to this system is keeping the TEG supplied with gas, but with consumption at 1.5 gallons of propane per day a 250 gallon tank can last nearly 6 months.

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Monitoring, Telemetry

After weeks of fabrication, the first of two PIT tag antennas was delivered and installed within a water diversion tunnel on a Northern California stream. This first installation was for the smaller of the two tunnels (12ft. by 15ft.) that we will be monitoring. Constructed from fiberglass, Kevlar, and plastics, the antenna array consists of three antennas that bolt together and operate in unison. With the use of a crane and a highly skilled operator, the three sections were joined together on site and toped with a steel frame that holds the array in position.  The steel frame allows the antenna to be removed once it is completely submerged. With a total height of over 20 ft., the antenna array was lowered through an access hatch on the top of the tunnel. With no room to spare, the antenna array fit snugly within notches in the concrete walls of the tunnel. Once the antenna array was in place, the readers were connected and powered up. With nervous anticipation, the antenna array was tested for detection range and with sigh of relief they operated perfectly.

When designing and building PIT tag antennas off-site, it is impossible to anticipate how it will function in the intended location. Metal structures, such as rebar imbedded into concrete, and low frequency electronic “noise” from electric motors or electronic devices can render an antenna inoperable. Now that this antenna array is operational, we can’t wait for increased winter flows so we can start recording tagged fish.

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Telemetry, Vaki Riverwatcher

On a recent visit to the Robles Fish Passage Facility in Southern California we noticed that Casitas Municipal Water District (CMWD) had enhanced their VAKI Riverwatcher system. Not only had they constructed an impressive crane to hoist the Riverwatcher and passage chute in and out of the fish ladder, they had also fitted their passage chute with a Passive Integrated Transponder (PIT) tag antenna. A PIT tag is a small radio transponder that will transmit a unique identification code when it is within range of an antenna sending out the correct radio frequency. We recently described a supersized PIT tag antenna array that we designed and constructed for diversion tunnel. When a fish carrying a PIT tag swims through the passage chute at the Robles facility, it isn’t just counted, measured and video taped, it also transmits valuable information about its past. Using the unique identification number, the fisheries biologists at CMWD can determine when the fish was tagged, what size it was when it was tagged, where it was tagged, when it was last detected and other relevant information.  It is always interesting to see how others have set up their fish counting devices, and the dual Riverwatcher-PIT tag system seems like a valuable asset.

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Fabrication, Monitoring, Telemetry

Last month we mentioned we were in the process of building a large, remote antenna array designed to detect and record passages of fishes and turtles tagged with Passive Integrated Transponder (PIT) tags. As our FABLAB crew can attest, many hours were spent constructing these supersized antennas.

With required dimensions in hand, we started by constructing the fiberglass shell. Since this array of three antennas will have to withstand high flows within the diversion tunnel, we used a curved mold to make the cross members of the antennas as hydrodynamic as possible. Once the rectangular shells of the antennas were produced, the electrical wiring was added. It only takes a couple wraps of a small gauge copper wire to produce the magnetic field required for charging the small PIT tag transmitters. After carefully positioning the antenna wire, we connected it to a tuner and tag reader from Oregon RFID, and then tested it for function and tag detection rate. With the electronics completed, more layers of fiberglass and Kevlar® (a high-strength, synthetic material) were applied and sanded until the desired shape and strength was achieved. Now that they are fully assembled and tuned, the three antennas can detect and record any tag passing through the center of the array. Watch for an update once this antenna is installed inside the subterranean tunnel and exposed to the full force of winter flows.

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Telemetry

People frequently come to us with new or challenging circumstances for research in aquatic environments, and that is certainly the case with one of the projects we are currently working on. In this situation, we want to use Passive Integrated Transponder (PIT) tags to detect and record presence of individual fish and turtles that may become entrained into a diversion tunnel. This is quite a tunnel, measuring about 12 feet wide, 15 feet high, and nearly 20,000 feet long. Detecting entrainment in this tunnel is further complicated by the fact that anything installed inside the tunnel will be inaccessible for a year and must be robust enough to withstand the pressure of water flowing up to the roof.

We opted to build an array of fiberglass PIT tag antennas that will transmit detection data to a land-based receiver. Unfortunately, making a single antenna around the tunnel perimeter is infeasible because of the limitations of the technology. We decided three stacked antennas would give us the best detection rate. When working with PIT tag antennas metal isn’t an option, yet we must construct them strong enough to withstand the force of water and debris they may encounter. We are currently fabricating PIT tag antennas from a mix of fiberglass and Kevlar to be ready for installation and testing next month. Watch for more posts on the fabrication and installation of these antennas for this challenging tunnel application.

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Telemetry

The concept of tracking fish is nothing new to anglers and fisheries scientists. For as long as we have been relying on fish as a source of food, we have wanted to know where they are and how to catch them. As fisheries scientists, understanding movement patterns on a finer scale is important in many ways. Acoustic telemetry can provide valuable information on the behavior and survival of individual fish, since every tag has its own predetermined signal that is emitted and the frequency is received and recorded by receivers (hydrophones). With the use of acoustic (or ultrasonic) tags, we can determine the type of habitat the tagged fish prefers, which route it will likely take and where hotspots for predation occur in the region.


Figure 1. Timeline of acoustic tagging projects in the Sacramento  (North Basin) – San Joaquin Delta (South Basin).

The first known use of acoustic telemetry in the Sacramento – San Joaquin Delta was a 1964 study on adult Chinook salmon. The tags were large (up to 4 inches long) and were attached externally near the dorsal fin of adult fish. In recent years, tagging fish with acoustic tags has become more popular because tags have been made much smaller (under 0.5 inches) and can be surgically implanted in juvenile fish (Figure 1). As more fisheries researchers began utilizing acoustic telemetry technology, hydrophones became more widespread in the Delta. Soon, it became evident that collaboration would be beneficial and cost effective; thus, in 2007 the California Fish Tracking Consortium was developed to facilitate sharing acoustic telemetry information. There are currently over 300 hydrophones that range from the Sacramento River in the north (near Redding), down to the most southern part of the San Joaquin River (near Fresno) and out to the Golden Gate Bridge (Figure 2).


Figure 2. Map of acoustic hydrophones in California’s Central Valley. Hydrophones are identified as Vemco or HTI and either California Fish Tracking Consortium or non-consortium.

Some acoustic telemetry studies focus in on a fish’s route selection and probability of survival, which could include predation rates, while others concentrate on determining a fish’s behavior in relation to specific structures or facilities. In the case of the Vernalis Adaptive Management Plan (VAMP), an acoustic telemetry study evolved over time into a study that incorporates multiple factors that affect salmon survival. In the Delta, there are multiple routes to the sea, and the route that the fish chooses can affect its chance of making it to the ocean. Initially, the goal of VAMP was to estimate survival and route selection of Chinook salmon smolts at different flows and exports in the presence or absence of the head of Old River barrier (HORB), but as the study progressed it was apparent that factors other than flows and exports were important. During the VAMP study, immobile tags (tags no longer moving because the fish is dead) were frequently found in places that were well known for good bass fishing. Furthermore, some tagged smolts suddenly developed behaviors indicative of predators (e.g., milling around in one spot for long periods of time, or making long-distance movements back upstream), indicating that predation upon Chinook salmon smolts may be more prevalent than previously presumed. When predatory fish tagging was added to the program it was discovered that striped bass, black bass, and other predators were milling around the state and federal diversion facilities where fish are salvaged and trucked downstream before entering the pumps.

The use of acoustic telemetry in the field of fisheries has significantly increased our knowledge of fish behavior. The majority of the tags that have been used in the Delta are for tracking salmonids and a smaller portion of tags have been used on sturgeon. The results from salmonid acoustic studies have led many to believe that predation by non-native fishes is one of several factors in the decline of native salmonids in the Delta. As a consequence, more acoustic tagging has been conducted on predatory fishes to better understand their habits and how we can protect native fish populations.

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Telemetry

The use of acoustic telemetry to monitor fish movements, behavior and survival has quickly gained popularity among fisheries researchers in California’s Central Valley within the past 10-15 years.  Primary fish species involved in acoustic telemetry studies are anadromous fish such as Chinook salmon, steelhead and green sturgeon as well as predatory fish such as striped bass.  Acoustic tags and technology allow researchers and managers to determine the numbers of tagged fish that migrate through specific routes, reach-specific survival rates, and movement rates.  Acoustic tags emit a pulse of sound at certain frequencies and in certain sequences (in milliseconds), that allow automated listening stations to hear the tag, then decode a unique tag ID.  The typical research plan typically consists of tagging a batch or batches of fish, releasing them, and tracking their movements either with fixed listening stations or hydrophones or through mobile surveys.

Many different groups (state and federal agencies, consulting firms, and universities) are conducting acoustic telemetry research.  In 2012, most research is being focused on Chinook salmon and steelhead smolts, and in particular, their survival rates through certain areas of the Delta and lower portions of tributaries that flow into the Delta.  With better knowledge of survival rates and movement patterns of Chinook and steelhead smolts through the Delta, water-export facilities can be operated with less risk to threatened and endangered species.

As you can see, our boat is nearly overloaded as we deploy an HTI acoustic array system in the lower Sacramento River to evaluate juvenile Chinook migration and survival.

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Field Crew, Telemetry

Perhaps we should give Mike Rowe from the Discovery Channel’s Dirty Jobs a call to help us with this project. This technician is sanding a PIT tag antenna cast in resin to get a good fit and a clean, smooth finish. One advantage of using half-duplex (HDX) PIT tags is the flexibility to custom fabricate antennas for many applications. This antenna was specifically designed to operate on the bottom of a round aquaculture tank in order to detect and log tags that fall to the bottom. It will be used in a study to evaluate the rate at which a tag passes through a striped bass’ digestive system. The results will help us understand what happens when a tagged salmon is consumed by a predator during salmon telemetry studies. Striped bass will be fed a salmon smolt with a surgically implanted tag and researchers will record how long it takes until the tag is defecated. It appears that this is a dirty job on many levels.

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Telemetry

FISHBIO, along with more than twenty researchers from the U.S. Bureau of Reclamation (USBR) and non-governmental organizations, traveled to Denver, Colorado this week to participate in a passive integrated transponder (PIT) tag training workshop. The classroom portion of the training was lead by Warren Leach from Oregon RFID. Warren not only sells cost effective PIT tags and equipment, but also provides outstanding technical support for people building their own passive interrogation systems. Warren did an excellent job providing training on the theory behind FDX and HDX technology (see PIT Tags: The Full Story) and providing practical design applications.

Vince Tranquilli from Oregon Department of Fish and Wildlife (ODFW), who has extensive experience constructing PIT tag antennas for fisheries research, led the participants in the many nuances of designing and building stream-width passive interrogation systems. In the photo above, Vince demonstrates resonance tuning of an 8 ft by 8 ft antenna fabricated out of a fine gauge copper wire. With some creativity, and a lot of patience, PIT tag antennas can be fabricated for many applications. Watch for more on the use PIT tags in future posts.