FISHBIO’s cross-cultural environmental education program in the United States and Southeast Asia helps students appreciate the importance of fish and rivers in their local communities and abroad. FISHBIO works with K-12 classrooms in schools in Chico, Oakdale, and Modesto, California, and schools in Vientiane, Lao PDR. The program includes presentations and classroom activities related to rivers as important fish habitat, including the Sacramento–San Joaquin River system and the Mekong River, as well as the lifecycle and migrations of Chinook salmon (Oncorhynchus tshawytscha) and the Mekong giant catfish (Pangasianodon gigas). Students in Lao PDR completed projects related to migratory Mekong fish species that were presented for a school event as part of World Fish Migration Day. FISHBIO also coordinated an international letter exchange among classrooms, and has created a website for the program at http://www.threeriversprogram.com/.