In many areas of Laos, fish stocks are declining as a result of overfishing, land use changes, environmental disturbances, and development activities. These losses in the capture fishery have prompted growth in aquaculture production as a means of compensation. FISHBIO visited official government hatcheries in two northern Lao provinces to review project operations and provide feedback and recommendations for operational improvements. Both facilities breed a combination of native and non-native fish species and sell them as fingerlings to local farmers and conservation projects. At each facility, FISHBIO met with local authorities, managers, and residents to obtain site-specific knowledge prior to conducting on-the-ground assessments. We then assessed fish pond and breeding tank design, breeding methodology, and broodstock management. We also conducted water quality testing at the fish ponds to examine the baseline environmental conditions at each hatchery.